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Reverse cholesterol transport: Novel insights
Prof. dr. Bert Groen
Universitair Medisch Centrum Groningen
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Definition of reverse cholesterol transport (RCT)
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Simplified scheme of cholesterol homeostasis
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Classic scheme of reverse cholesterol transport
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Disruption of Acba1 should have a drastic effect on fecal neutral sterol excretion
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Disruption of Abca1 in mice has no effect on neutral sterol excretion in feces
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Determination of tissue cholesterol efflux using 13C-Cholesterol infusion (Kinemed method)
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Classic scheme of reverse cholesterol transport
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About 40% of tissue cholesterol efflux in humans is HDL dependent
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Summary I
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Intestine perfusion
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Intestinal perfusion to measure direct cholesterol secretion
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Trans Intestinal Cholesterol Excretion (TICE)
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Transintestinal cholesterol secretion (TICE)= transport directly from blood to intestinal lumen
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In vivo determination of TICE in mice is straightforward.
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Summary II
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In vivo determination of TICE in humans is complicated because biliary cholesterol output has to be quantified
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Estimation of biliary cholesterol output
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Study protocol
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Simplified model of cholesterol fluxes
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Summary III
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